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MATERIALS AND METHODSMaterialsGR (type IV, from Baker's yeast), GSSG, NADPH, DTNB, GdnHCl, and HEPES were from Sigma Chemical (St home health health care equipment .
The mean residue ellipticity of the recombinant protein was similar to that of the wild-type, suggesting that there are no major structural changes between the native glycosylated and recombinant nonglycosylated proteins (Fig home health health care equipment .
The relationship observed between reduced protein flexibility and increased thermal stability is in line with other experiments on trehalose-coated myoglobin (56) and on lysozyme in glycerol (57,58) home health health care equipment .
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The translational entropy of counterions and a hydration effect play important roles in this process home health health care equipment .
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1 C), yet it shows hysteresis and scan rate effects characteristic of thermodynamically irreversible reactions, as indicated by the CD and light-scattering data in Fig home health health care equipment .
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