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0 adjusted using DCI or NaOD
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In our EV model, there is only one free parameter, namely the exponent n
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thus indicating that the helical content of EIC^sup sc^ increased
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The plots can be interpreted in analogy to Guinier plots of polydisperse dilute solutions: In SAS experiments, linear regimes can be assigned to populations of distinct radii of gyration R^sub g^, provided Q*R^sub g^ Whereas at low temperatures, intensities decreased linearly over the whole Q-range measured for all samples, a more or less pronounced kink appeared in the Guinier plots above ~200 K (Fig
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, limited to the active site), concern low-frequency collective modes below the sensitivity of the technique used and/or present only on a longer timescale (> 1 ns), and not directly related to fast thermal fluctuations
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27 mN for these two temperatures
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Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, Thioflavine T
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Fluorescence accessory was used to record 90° light scattering simultaneously with the CD
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A total of seven P
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Using the Boehlert and Yoklavich (1984) equation; (stage duration = O
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Because of the low number of females with developing embryos collected in 1996 and 1998, 19 and 4 females, respectively, and based on the results of prefertilization females, all data were pooled and fitted with one model (Fig
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We thank Dagmar Klostermeier for assistance in preparing some of the hairpin ribozyme constructs
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2a) and the cells were small, approximately 10 µm in diameter, and laden with lipid filled vacuoles (Fig
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--Pharmaceutical Sciences* CALIFORNIAUniversity of California, San Diego School of Pharmacy and Pharmaceutical Sciences M
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The reactions to stressors section is comprised of four categories (physiological, emotional, behavioral, and cognitive)
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. In brief, all FTIR experiments were performed on a Perkin-Elmer Spectrum 2000 spectrometer (Wellesley, MA) equipped with a dTGS detector
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.Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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.The peak position for the SL 3WJ remains in the low-FRET region of the histograms, independent of the Mg^sup 2+^ concentration, indicating that this ribozyme construct is incapable of folding to a compact structure (Fig
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. The trFRET method permits the spectroscopic resolution of an equilibrium mixture of alternate RNA conformers from an analysis of the effect of energy transfer to the acceptor on the fluorescence decay kinetics of the donor (Klostermeier and Millar, 2001c)
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