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We've been able to implement process changes that have eliminated finger-pointing and barriers, specifically in our coding, dictation, and chargemaster areas
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. By using a combination of fluorescence and circular dichroism spectroscopies, thiol group titration, and size-exclusion chromatography we have detected the existence of partially folded intermediate states in the unfolding of GR
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. Precisely how many and which nonnative local contacts are to be broken is determined by the native-state topology and hence the specific amino acid sequence
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. Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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. Comparison of the liquid-liquid and literature solid-liquid coexistence curves showed these solutions to be supersaturated and the phase separation to be nonequilibrium in nature
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. Phase separation occurred in the metastable region
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. Source of variationdf Sum of squaresMean squareAbsolute fecundity (basedon prefertilization oocytes)Equality of slopes 2105,34752,674Error1604,564,32028,527Equality of elevation2168,54484,272Error1623,459,048 288,254Relative fecundity (basedon prefertilization oocytes)Equality of slopes 2 259
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