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The former corresponds to that of the intermediate, whereas the latter peak represents the partially denatured population kaiser health insurance california .
As a result, for each sequence, we generated an ensemble consisting of 10^sup 4^ uncorrelated conformations kaiser health insurance california .
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To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former kaiser health insurance california .
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Solvent stabilization by salting-out ions is a consequence of a decrease in free energy of the native state, due to both entropic and enthalpic contributions; in particular, the effect on hydration water is expected to be mainly entropic, whereas the observed decrease in protein MSDs (44) indicates a tighter packing of the apolar residues in the core of the protein as suggested for hydrophobic stabilization by calorimetry experiments (59) kaiser health insurance california .
The ratio f is usually dominated by the exponential factor, so that the right-hand term in Eq kaiser health insurance california .
7, with background corrected from unbound probe kaiser health insurance california .
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In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram kaiser health insurance california .
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67, respectively; again, the effect was significant, F(1, 11) = 2525 kaiser health insurance california .
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