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. To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former
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. Solvent stabilization by salting-out ions is a consequence of a decrease in free energy of the native state, due to both entropic and enthalpic contributions; in particular, the effect on hydration water is expected to be mainly entropic, whereas the observed decrease in protein MSDs (44) indicates a tighter packing of the apolar residues in the core of the protein as suggested for hydrophobic stabilization by calorimetry experiments (59)
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. In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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