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. Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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. RESULTS AND DISCUSSION The thermal dependence of ACYI activity was determined by measuring the catalytic activity of the samples equilibrated at given temperatures tor 30 min
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. Instead, they are soft ellipsoids that show large correlated fluctuations about mean values for R^sub g^ and δ
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. Since the folding process is complex in multidomain proteins, where each domain may be able to refold independently and interdomain interactions may affect the overall folding process, EI can be used, in addition, as a model protein to improve our knowledge of protein folding and assembly of multidomain-oligomeric proteins
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. Protein concentration was 4 µM
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. An important time window in the picosecond-to-nanosecond time range is occupied by atomic thermal fluctuations, which have been extensively investigated by molecular dynamics (MD) simulations (2), and by various experimental approaches including neutron scattering (3,4) and nuclear magnetic resonance (NMR) (5)
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. Unlike at 60°C, the fibrillization at 70°C appears to be complete within ~24 h (Fig
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. The rate-determining "gutter" dimensions lie orthogonal to other "zipper" dimensions describing the local formation of secondary structure, that are characterized by a continuous folding funnel
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. Formally, folding can be viewed as a symmetry-breaking operation wherein the dilatation symmetry characteristic of the denatured-state (EV limit) ensemble is broken by breaking or disrupting the requisite number of nonnative local contacts
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. 5) are identical for the folded native and soman-inhibited enzymes
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. Tjandra and co-workers (3) have shown, via ^sup 15^N-relaxation at multiple fields, that in free CaM the two domains undergo a slow "wobbling" motion on a timescale of ~3 ns, apparently not evident in target-bound CaM
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