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In addition, the peak corresponding to tryptophan emission displayed a significant red-shift to [lambda]^sub max^ ~ 334-339 nm managed health services .
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On the other hand, at acidic pH, EI^sup sc^ lost its tertiary structure and solvent-exposed hydrophobic patches, as suggested by 1), the changes in the fluorescence intensity (Fig managed health services .
Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig managed health services .
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Samples containing 1-anilinonaphthalene-8-sulfonate (ANS) and Bis-ANS were excited at 290 nm, 370 nm, and 403 nm managed health services .
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