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. Apart from its crucial functions in intracellular amino acid metabolism, ACYI also has substantial commercial importance for the optical resolution of N-acylated DL-amino acids
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. org and during the 3rd International Symposium on Milk Genomics & Human Health, which will be held September 19-21, 2006, in Brussels, Belgium
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. We can speculate, however, based on the modeled three-dimensional structure of the EIC^sup sc^
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. Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe
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. To examine the reason for this factor, we compared T^sub 2^, the spin-spin relaxation times, of the methylene peak at 1
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. Picoliter ^sup 1^H NMR Spectroscopy
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. Tkác, and R
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. We find that residues with significant exchange broadening (Table 1) group in a model structure of the CaM:SEF2-1mp complex into three regions: i), the CaM dimerization interface, i
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. For AHS both approximate analytic (71) and recent machine calculations (20) indicate that to a very large extent the metastable liquid-liquid coexistence curve lies below the percolation line, and so particle gel formation rather than liquid-liquid separation is predicted
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. Therefore, the difference between DCVJ and ThT is that ThT does not fluoresce in the presence of protofibrillar amyloid of TTR at pH 3
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. Gα^sub s^ was purified from SP9 cells by the same methods used for Gα^sub q^ purification (see Runnels et al
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. Larger proteins may do this in an increasingly hierarchical way
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. Funnels, pathways and the energy landscape of protein folding
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. Even though the abundance and distribution data used in our study came from cruises that targeted other species, they provide satisfactory spatiotemporal coverage
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. Ten percent of the otoliths were randomly selected for a second reading to ensure consistency in interpretation of annuli
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. Continuous cultures of fused cells secreting antibody of predefined specificity
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. These observations in the IR spectra coincided with the deduction based on DSC results that the thermal unfolding of ACYI is an irreversible process accompanied with protein aggregation
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. Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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. Similar results were obtained for all other two-state protein sequences shown in Table 1
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. Tetrameric native and soman-inhibited human butyrylcholinesterase (HuBChE) as well as native dimeric Drosophila melanogaster acetylcholinesterase (DmAChE) hydrated protein powders were examined
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. In the absence of DNA, the basic sequences loose their well-defined secondary structure (14)
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.Nuclei that are influenced by chemical exchange have been shown to correlate with residues known to be critical for protein interactions and enzymatic activity (39,40, 41,42,43,44)
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. The sliding process is likely to occur on a nanosecond timescale, although CaM:SEF2-lmp interactions may slow down this process in some instances
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. High-resolution probing of local conformational changes in proteins by the use of multiple labeling: unfolding and selfassembly of human carbonic anhydrase II monitored by spin, fluorescent, and chemical reactivity probes
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