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. To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former
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. All fluorescence spectra were corrected for background scattering as measured with pure buffer
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. For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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. In contrast to high temperatures the presence of GndHCl efficiently suppresses aggregation
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. In C3A/C26A the elimination of the superficial C3-C26 disulfide bridge, linking the ends of strands β land β2, has little effect on the compactness of the protein core and, judging from the phosphorescence properties of W48 embedded in it (18), internal motions remain restrained
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. Even though Gα^sub i^(GDP) did not significantly affect the PLCβ^sub 2^-Gβγ equilibrium, it unexpectedly resulted in rapid deactivation of the PLCβ-Gβγ complex when PLCβ activity was monitored (Runnels and Scarlata, 1998)
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