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"After some opening crowd-pleasers, Cash strums his guitar and addresses his audience in a no-nonsense tone that immediately gives them back some freedom of choice: I tell you what:
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. Thus, the relationship between thermal atomic fluctuations and functionally important motions is not straightforward
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. Pressure and propylene carbonate denaturation of native and "aged" phosphorylated cholinesterase
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. Below the short Newtonian plateau, the viscosity increases with decreasing shear rate
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. Structural basis of the Tanford transition of bovine beta-lactoglobulin
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. The binding affinity was compared between the different probes defining an intensity 50 value, I^sub 50^, being the half height of the maximum emission intensity, corresponding to an apparent dissociation constant
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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. This approach suggests a natural interpretation of Φ-values >1, such as recorded in acylphosphatase (Chiti et al
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. We selected a dissolving temperature of 70°C
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