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The fluorescence intensity of Bis-ANS decreases as the A-state aggregates, well in accordance with hiding of accessible surface area in contrast to the behavior of ANS, likely due to the fact that Bis-ANS is more bulky national institute of health bethesda md .
Additionally, PLCβ^sub 2^ and β^sub 3^ can independently be activated by Gβγ-subunits as well national institute of health bethesda md .
Fluorescence measurements and analysisFluorescence measurements were performed on an ISS spectrofluorometer (Champaign, IL) national institute of health bethesda md .
If this idea is correct, then the volume associated with Gβγ activation of PLCβ corresponds to the difference in volume changes for PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), ranging from ΔV ~3 to 15 ml/mol (Table 1) national institute of health bethesda md .
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1 and then normalized by dividing it by the number of atoms of each residue national institute of health bethesda md .
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