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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. RESULTS Structure and stability of EI^sup sc^ in the absence of effectors We carried out KI quenching experiments at different protein concentrations to test whether 1 )
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. Most of these difficulties are not present if λ^sub max^-signals are employed to characterize unfolding transitions, although these signals can give a skewed assignment of the population of microstates, because a priori the λ^sub max^-signal is not proportional to the population of the states
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. After 2 h, only very small aggregates (400 h), the protofibrils have increased in length to 100-200 nm, and the spherical clusters have decreased in size to 20-30 nm in diameter (Fig
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