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Some of the center's work is devoted to such laudable causes as wiping out the parasitical guinea worm
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. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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- William Jefferson's freezer, he became the rightful target of a storm of bipartisan anger
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. Folding/unfolding can be followed by measurements of biological activity and/or by a variety of spectroscopic methods
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. Second, an equal volume of 2-butanone was added and mixed thoroughly
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. More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. Deviations from the accurate field-theoretic exponent of v = 0
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.34^ for folded proteins (66), the scaling law itself does not restrict folded proteins to be spherical globules
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. GndHCl-induced unfolded states show for all α-amylases emission spectra with λ^sub max^ of ~348-349 nm which is nearly the value one would expect for fully accessible tryptophan, namely 350 nm
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. Most interestingly, they found that a substantial part of the AChE molecule is involved in the gorge opening and not just a few gorge residues
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. Processive enzymes, when sliding along their targets (50), have the closest similarity to CaM:SEF2-1mp binding
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. The second virial coefficient is negative in this pH region, indicative of there being short-ranged attractive forces
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. Bound to aggregated TTR, the fluorescence lifetime of both components have increased and the relative amplitude of the slower component has increased (Table 3), well in accordance with the massive increase in quantum yield compared to when bound to native TTR (Fig
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. Native and nonnative interactions along protein folding and unfolding pathways
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.Comprehensive structural insight is, however, limited by the intrinsic flexibility of the system
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. The connectivity between pharmacies can be established over the Internet or via the chain's proprietary network
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