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. Conformational change in the C-terminal domain is responsible for the initiation of creatine kinase thermal aggregation
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. (34)[FIGURE 3 OMITTED]Efferocytosis also increases the production of secretory leukoprotease inhibitor, which is an antiprotease with activity against elastase and cathepsin G
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. Although PPA exhibits a strong aggregation for the thermal unfolded state, we do not observe any dependence of the magnitude of the blue shift on the heating rate
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. The former may reflect the relative ease with which a smaller protein with fewer lipid-binding helices can dissociate from the disk as compared to a larger protein with multiple, possibly cooperative lipid binding sites
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. , 1999), much slower folding is generally observed, especially for large RNAs with complex tertiary structures (Treiber and Williamson, 1999, 2001)
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. The loss of these specific tertiary docking interactions makes these SL constructs mimics for the undocked state of the corresponding WT ribozymes
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. Hence, quasi-docked and specifically-docked ribozyme species are readily distinguished in our diffusion spFRET experiments
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