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. 5 and 3 M GdnHCl corresponded to expanded predissociated dimers and molten globule monomers, respectively
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. For example, the stability of the porcine odorant hinding protein is 4
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. American Institute of Physics, New York
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. FTIR The protein was speed-vacuum dried (Speed-vac rotatory evaporator, Savant Instruments, Farmingdale, NY) and dissolved in the deuterated buffer containing 10OmM NaCI
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. The methine peak at 5
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. Addition of 100 mM NaCl to the acid unfolded TTR monomers will immediately shield the positive charges and initiate a conformational change into a so-called A-state (Lai et al
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. Vertical dimensions of the fibrillar aggregates were obtained from individual cross-section profiles generated by the AFM's accessory data analysis software
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. 9 nm in diameter) partially unfolded spherical oligomers are apparently capable of merging into the amyloid precursor
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. Funnels, pathways and the energy landscape of protein folding
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. 7 mm BL, at a relatively large size range compared to that for Parapercis spp
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. For links to both of these articles, visit www
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. Furthermore, the relatively long side chains of both residues might also tolerate a higher degree of flexibility despite the presence of a salt bridge
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. When the methanol was added, the IPP toner settled at the bottom of the flask
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. 8 × 10^sup 3^ s^sup -1^, respectively
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. Beyond this length scale, all internal distances scale with sequence separation in accordance with the standard power law for proteins in a good solvent
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. For experiments involving PEP, control scans were acquired with PEP added to buffer versus buffer in the reference cell, and baselines were found to be the same as in buffer-buffer baselines, suggesting that under these conditions hydrolysis of PEP during heating was not significant
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. (56) This effect of lovastatin stems from its disproportionate effect on suppressing the prenylation of RhoA over Rac-1
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. Using polyethylene glycol solutions as a model of a crowded cell environment, we studied DNA compaction by alkali metal salts LiCl, NaCl, KCl, RbCl, and CsCl, and found that all of these monocations promote DNA compaction
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.PLCβ enzymes are part of a larger family of mammalian PLCs
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. Therefore, we characterized the membrane binding behavior of PLCβ and G-protein subunits before protein-protein association studies
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. CPM-labeled proteins were excited at 385 nm and scanned from 420 to 540 nm
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. Denaturant-induced movement of the transition state of protein folding revealed by high-pressure stopped-fiow measurements
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