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. Dielectric spectroscopy provides direct information of the dynamic behavior of molecular dipole orientation in solution
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. Thermodynamics and kinetics of unfolding of the thermostable trimeric adenylate kinase from the archeon Sulfolobus acidocaldarius
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. A promising approach to elucidate the effect of aggregation in more detail and maybe on a quantitative level would be the use of encapsulation techniques
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. Establishing quantitative relationships between thermal fluctuations and functional motions is challenging, taking into consideration the vast variety of protein functions (synthesis, catalysis, electron- and proton transfer, etc
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. In vitro mutagenesis and expression in Xenopus oocytes
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. 1 is used) assuming the apparent specific volume of the protein phase to be 1
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. Biomolecular hydration: from water dynamics to hydrodynamics
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. Li+ shows moderate chemical potential in PEG 3000 but is the weakest DNA compaction agent in PEG 10000
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. Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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. Interestingly, a lack of enhanced membrane binding was also observed for the nonspecific PLCβ-membrane association, suggesting that the enzyme packs efficiently with the membrane interface
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. The solvent dye is well distributed throughout the particles
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. Timing of parturition was estimated by microscopically determining embryo development stages for all females with fertilized eggs following Yamada and Kusakari's (1991) stages of embryonic development for kurosoi (Sebastes schlegeli) modified to reflect the gestation period of 37 days for black rockfish (Boehlert and Yoklavich, 1984)
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. Additional funding was provided through Hatfield Marine Science Center scholarships: the 1996 Barbara Schwantes Memorial Fellowship; the 1997 Mamie L
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. Mito (1960, 1979) and Ikeda and Mito (1988) developed methods for identifying pelagic fish eggs based on morphological features
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. Although only late stage eggs were used in this experiment, early stage eggs are also detectable because the antibody recognized both stages of P
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. It was removed intact and without complication
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. (11-13) The potential complications of retained fragments include lead poisoning, fistula formation, recurrent infections, and secondary hemorrhage
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. Given the racial overtones of the Tulia cases, it's worth noting that the officers and informants implicated in the Dallas scandal are Hispanic, like their victims
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.Collectively, we have worked with blind cave organisms for more than eighteen years, and one of us (Luis Espinasa) was working with blind cave fish in Jeffery's laboratory shortly after the publication of the momentous work on lens transplants
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. In the interest of clarity, we show data for the sequence of ubiquitin
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. The mean residue ellipticity, [Θ], was obtained from the raw ellipticity data, Θ, as described (23), and all spectra were corrected with the proper baseline
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. Molecular confinement influences protein structure and enhances thermal protein stability
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. The quasi-docked conformation interconverts rapidly with an extended structure, giving rise to continuous peak shifts with added Mg^sup 2+^, as well as pronounced asymmetry and broadening in the FRET efficiency histogram at high Mg^sup 2+^ concentrations
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