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Steady-state ANS binding ANS binding was detected by collecting fluorescence spectra at different pH in the presence of 100 µM of dye
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Evidence of a role of tumor necrosis factor alpha in refractory asthma
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Outside of these conditions, proteins exhibit denatured and structurally unfolded states
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For instance, the barnase (45) and oxalocrotonate tautomerase (46) bind specifically to their rigid target to form a specific complex with essentially one overall conformation
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As the associated shear stress was [much less than]50 kPa, we ascribe this behavior to being equivalent to the η^sub 0^ of hard sphere colloidal suspensions
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5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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Formation of an amyloid reflects an alternative to the native packing conformational struggle of a polypeptide chain to 1), reduce its surface accessible area; 2), saturate hydrogen bonding; and 3), reach an alternative non-native global free energy minimum (Gazit, 2002)
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Effect of denaturant and Chevron plotsAs central a tool to the experimental elucidation of folding kinetics as the protein engineering extraction of Φ-values is the tracking of folding rate with denaturant concentration
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2 B), where a subspace of the conformational space of the free molecule is sampled in the complex state
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Particle formation depends on various solution parameters and cooling conditions
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However, eggs of some sparids, such as Acanthopagrus schlegeli, Sparus sarba, and Dentex tumifrons are extremely difficult to distinguish from eggs of P
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