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. 6), although isothermal melt done with rSBA at this protein concentration still shows a three-state unfolding profile
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. In general, the presence of Mg^sup 2+^ +, PEP, or both effectors together had a minor effect on the thermal stability of the protein as judged by the slight changes in T^sub m^ (the ΔΔT^sub m^s from DSC measurements equal + 1
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. A similar reasoning can be used to explain the outcome of the effectors
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. Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli
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. Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte
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. 3 mm, pixel size = 18 µm × 18 µm, slice thickness = 200 µm, matrix (MTX) = 128 × 128, spectral bandwidth (SW) = 25 kHz, repetition time (TR) = 2000 ms, echo time (TE) = 10 ms, number of excitations (NEX) = 8, acquisition time (T^sub acq^) = 26 min
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. Figure 10 shows the microstructures of both Sn-Pb and Sn-Ag-Cu-Bi solders with Cu and Ni substrates
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. Sample solutionsSolutions for microscopic observations were prepared by mixing PEG 3000 or PEG 10000 in twice-distilled water until dissolution, adding T4 DNA with further mixing and incubation for 30 min, adding DAPI with further mixing and incubation for 30 min, and finally adding alkali chloride solution and letting the sample solution sit for 1-2 h before observations
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. The distribution of DNA lengths in the final globule conformation is very narrow, with an average length of ~0
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. 8 µM for ThT (Fig
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. Correlation between the protein size and disk stability may result from enthalpic and/or entropie effects
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. This is not yet reachable for proteins with the current computation timescale of nanoseconds
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. --Pharmaceutical Sciences Health Services AdministrationUniversity of Nebraska College of Pharmacy M
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. Similar effects have been reported in horse racing, where people take greater risks later in the day in an effort to win back their losses (Johnson & Bruce, 1993; Kopelman & Minkin, 1991)
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. This type of effect has been observed i n situations in which there is only a fixed period of time to gamble (i
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. Plants beneath those trees include Curtiss' milkweed, dwarf pawpaw, shiny blueberry, and numerous kinds of grasses
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.MATERIALS AND METHODSMaterialsGR (type IV, from Baker's yeast), GSSG, NADPH, DTNB, GdnHCl, and HEPES were from Sigma Chemical (St
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. The wettability assessment parameters such as contact angle (θ^sub c^) and maximum wetting force (F^sub w^) were documented for three solder bath temperatures with three commercial fluxes, namely, no-clean (NC), nonactivated (R), and water-soluble organic acid flux (WS)
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. On the other hand, the high fluxing activity of WS flux provides adequate wetting for Ni substrate; even the wettability of Sn-2
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.The three probes which show increased fluorescence in correlation to the TTR misfolding reaction reveal a fluorescence signal during binding to different species
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. It is also likely that a significant proportion of such tailored nonnative interactions that we envisage guiding the search will be increasingly near-native as the search proceeds
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. An additional example of tuned nonnative interactions guiding a folding pathway occurs in the rather larger Phage 22 Tailspike protein (Robinson and King, 1997), where a nonnative disulphide bond controls the folding search
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. The first is that special tuning of nonnative interactions may contribute significantly to rapid folding; they stabilize the hypergutter-potentials that keep diffusive search dimensions under control
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.And in reflecting light from nearby stars, it exhibits a variety of colors that reveal much about its own composition as well as the influence of its surroundings, says David Malin of the Anglo-Australian Observatory in Epping, Australia
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., Northampton, MA), and the peak position (E^sup peak^) and relative areas of individual peaks were calculated from the fitting parameters
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. The ribozyme system is sampled at 40 times per measurement interval to simulate the effects of the threshold of 40 photons we used for the FRET analysis
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. For example, the ability to distinguish between one or two ribozyme conformers in the trFRET analysis is based on the goodness of fit obtained using unimodal or bimodal donor-acceptor distance distribution models
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