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6 (lower chromatogram) persists even after overnight incubation (data not shown), indicating that a small proportion of dimers is still in equilibrium with monomers at this concentration of denaturant student health insurance .
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Apparently, glycosylation promotes the protein with greater stability, which is considerable in its magnitude (9 kcal/mol) student health insurance .
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The impact of aggregation on fluorescence emission spectra To investigate how fluorescence emission spectra are affected by aggregation we measured the aggregation directly by the use of elastic light scattering and we analyzed unfolding transitions under different conditions which lead to a different extent of protein aggregation student health insurance .
A dependence on the number of the glycosylation sites was found for only a small percentage of the exchangeable amide H-atoms (65), the exchange rate decreasing with increasing glycosylation student health insurance .
Entropy Calculation of HIV-1 Env gp120, its Receptor CD4, and their Complex: An Analysis of Configurational Entropy Changes upon ComplexationHsu, Shang-Te DABSTRACTThe HIV-1 gp120/CD4 interaction shows a large, unprecedented entropy/enthalpy compensation, with the capacity to fine-tune recognition over a broad range of affinity student health insurance .
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Steady-state FRET methods were first used to monitor the docking of loops A and B during magnesium-induced folding (N student health insurance .
spFRET measurements and data analysisSingle-molecule measurements and data analysis were performed as described previously (Deniz et al student health insurance .
Moreover, the quasi-docked species observed in our study appears to form very rapidly, with a rate constant that is two to four orders of magnitude faster than the value reported for formation of the intermediate for the immobilized 4WJ ribozyme under similar conditions (Tan et al student health insurance .
Energetics of hydrogen bond networks in RNA: Hydrogen bonds surrounding G+1 and U42 are the major determinants for the tertiary structure stability of the hairpin ribozyme student health insurance .
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