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0, containing 1 mM EDTA and the indicated concentrations of GdnHCl
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Decreased apoptosis and increased activation of alveolar neutrophils in bacterial pneumonia
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15 M Tris-HCl, pH 8, followed by column chromatography (23)
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The viscous behavior of globular protein solutions will therefore be strongly influenced by the characteristics of this hydration layer
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The quenched Trp fluorescence in the presence of ANS and Bis-ANS (Fig
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Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II
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Kinetic Stabilization and Fusion of Apolipoprotein A-2:DMPC Disks: Comparison with apoA-1 and apoC-1Jayaraman, ShobiniABSTRACTDenaturation studies of high-density lipoproteins (HDL) containing human apolipoprotein A-2 (apoA-2) and dimyristoyl phosphatidylcholine indicate kinetic stabilization
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Fat crystallization requires about two hours, regardless of the cooling temperature
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Time-resolved FRET (trFRET) measurements were subsequently used to resolve and quantify the equilibrium distribution of extended and docked ribozyme conformers (N
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4 percent less than their counterparts at the associate professor level; and an average of 11
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ACKNOWLEDGEMENTSThe authors would like to thank Bradley Wood, Chrystal Pierce, Anette Herrera and Shoreh Biazar-Sadri for their help with data collection
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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. The recomhinant form also shows emission maxima at 327 nm for the native stale and 357 nm in the completely denatured state (Fig
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. The Kratky profiles reveal the presence of three distinct regimes for each sequence
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. More experimental details are found in Supplementary Materials (Table RS1)
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. The data are presented following the flow diagram of the PINATA script
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. It appears therefore that even for a homogenous insulin sample undergoing uniform temperature treatment, there is still more than one mode of superhelical assembly of protofilaments
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. The large number of these, of order N times greater than the number of native interactions, make them a likely candidate for information storage, as well as their natural propensity for kinetic control
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. Hence, the helical junction geometry strongly affects the tertiary folding of the hairpin ribozyme
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. Hence, this quasi-docked species must also form in the native 4WJ ribozyme, possibly as a precursor to the specifically docked native state (Fig
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