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. The MG concept was introduced to describe the properties of partially folded intermediate states sharing similar structural characteristics (i
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. Our ability to simulate mimics of denatured-state ensembles will allow us to ask precise questions about the role of the denatured state in the types of applications outlined above
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. FTIR spectra in the presence of the effectors were acquired and compared with those obtained in their absence to find out whether there were secondary structural changes upon binding
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. The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells
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. The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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. 5 nm, and appears to consist of joined segments ~153 ± 8 nm long
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. In consequence there are contributions to measured Φ-values that arise from this "preexponential" effect
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. 3 kJ K^sup -1^ mol^sup -1^ and ΔS^sup 2nd,b-f^^sub gp120,all^ = 0
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. 2] at room temperature for 15 minutes
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. Species MT-1 MT-2 MT-31 Pagrus major +++2+++3+++4+++5+++6+++7+++8+++9 Acanthopagrus schlegeli---10 ---11 ---12 ---13 ---14Acanthopagrus latus---15Sparus sarba ---16Dentex tumifrons ---17Paralichthys olivaceus ---18 ---19 ---20 ---21Engraulis japonica ---Table 4Reactivity of monoclonal antibody MT-1 to the pelagic eggsfixed with formaldehyde just after collection from WakasaBay
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. The sequences are 5' -Cy3 AAA UAG AGA AGC GAA CCA GAG AAA CAC ACG CC-3' (2WJ/3WJ/4WJ), 5'-Cy5 GGC GUG GUA CAU UAC CUG GUA CCC CCU CGC dAGU CCU AUU U-3' (2WJ), 5'-Cy5 GGC GUG GUA CAU UAC CUG GUA CGA GUU GAC-3' (3WJ/4WJ/4WJ single-loop (SL)), 5'GUC AAC UCG UUC GCdA GUC CUA UUU-3' (3WJ), 5'-GCA AGC CAC CUC GCdA GUC CUA UUU-3' (4WJ), 5'-GUC AAC UCG UGG UGG CUU GC-3' (4WJ/4WJ SL), 5'-Cy5 GGC GUG GUA CAU UAC CUG GUA CCC CCU CGC GAG CCU AUU U-3' (2WJ SL), 5' -GCA AGC CAC CUC GCG AGC CUA UUU-3' (4WJ SL) and 5'-Cy3 AAA UAG GCU CGC GAA CCA GAG AAA CAC ACG CC-3' (2WJ & 4WJ SL)
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. However, based on the observed FRET efficiency, the two loop-carrying arms appear to be relatively far apart in that species, approximately midway between the extended and docked conformers
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