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. The scaling exponent v [asymptotically =] 0
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. Understanding protein folding via free energy surfaces from theory and experiment
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. Details about site-directed mutagenesis, protein expression, isolation, and purification of mutants I7S, F110S, and C3A/ C26A have been described elsewhere (22,24)
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. Below we consider equations assuming fast exchange
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. Although the protein globule may carry a zero net charge, its surface charge distribution is heterogeneous and the net charge reflects the balance between positive and negative charges
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. The intensity of positive immunostaining was generally moderate but was not graded
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- "There is no formula for this," she says
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. Complexity is not always synonymous with energy expenditure
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. rSBA showed no change in either the fluorescence emission profile or the secondary CD profile
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. PyMOL molecular graphics system on World Wide Web URL: http://www
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. Kinetics of aggregation and gelation of globular proteins after heat-induced denaturation
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. Mass normalized fluorescence profiles are shown in Fig
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. The samples are denoted HuBChE-D^sub 2^O and Soman/HuBChE-D^sub 2^O, respectively
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. ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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. We find that Gα(GDP) significantly decreased the volume change for the CPM-PLCβ-Gβγ association (Table 1)
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.Comparison with apoA-1 and apoC-1To assess the relative stability of the DMPC complexes with various human apolipoproteins, we used DSC, CD, and light scattering to monitor heat denaturation of similar-size DMPC disks with apoA-1, apoA-2, or apoC-1 under otherwise identical conditions
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. This feature is consistent with interconversion between compact and extended structures, which we estimate takes place on the 100-µs timescale using a simple model for the peak shape
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