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. These results suggested that the active site of the enzyme might remain intact at temperatures below 65°C under our conditions, whereas the loss of activity at temperatures above 65°C might be due to heatinduced unfolding
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. It has been proposed that the catalytic domain contains a β-sheet sandwiched between α-helices and a second β-sheet located on the surface, whereas the dimerization domain consists of a β-sheet flanked on one side by two α-helices (17)
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. This work was supported by Projects CTQ2004-0771 ft from the Spanish Ministerio de Educación y Ciencia (MEC) to J
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. Compared to affinities of 0
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. The linewidths of the vegetal peaks were twice those of the animal peaks
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. It should be noted that the diminazene aceturate concentration we used for the cellular uptake experiment is relatively high (250 mM) but still not even half a percent of the concentration of water which dominates the MR signal
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. Although the misfolding and aggregation reaction is very complex and of multiple order, all kinetic traces could be fitted to a double exponential function which enabled comparison of kinetic parameters
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. The simplest explanation is that GDP-bound Gα^sub s^-subunits bind to Gβγ, but not necessarily at its primary interaction site
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. The entropie effect may result from the contribution of the cratic (mixing) entropy of protein dissolution to the entropy of disk denaturation
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. The spectra were recorded in a 0
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