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. Similarly, repetitive T-jumps with DMPC disks containing apoA-1 and apoC-1 show faster unfolding in the second and consecutive jumps (data not shown)
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. A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. 1, are turned off, we obtain Flory's freely rotating chain model (43), albeit with a constraint that the peptide units are all in a trans configuration
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. (32) The first pathway includes UNC-73, MIG-2, ced-12, ced-5, and ced-2 (representing the mammalian homologues TRIO, RhoG, ELMO, Dock 180, and CrkII, respectively)
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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. We have previously shown that Gα(GDP)-subunits cause de-activation of PLCβ-Gβγ without significantly affecting their association (Runnels and Scarlata, 1998)
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