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In addition, size-exclusion chromatography analysis indicated that the intermediates populated at 2
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Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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Amyloid fibrils from the viewpoint of protein folding
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Stability of EI^sup sc^ as monitored by DSC and thermal denaturations From the spectroscopic results above, it can be concluded that EI^sup sc^ acquired a folded conformation around physiological pH, and that this structure was basically unaltered (exeept for changes in the ellipticity, Fig
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In less-polar environments of the tryptophan chromophore the stokes shift will be less pronounced as compared to more-polar surroundings
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Most probable a well-pronounced aggregation of the thermal unfolded state contributes to the burial of tryptophan residues (discussion of this subject follows in the next section)
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NaCl suprapur was purchased from Merck (Darmstadt, Germany) and ANS from Sigma (St
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In an earlier NMR study we could conclude that the CaM:SEF2-1mp complex has a 2:2 stoichiometry, where two interacting CaM molecules bind one homodimeric SEF2-1mp (11)
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The interpretation of the RT^sup n^^sub 1^ versus NOE plots is straightforward
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After amplification and discrimination, signals were directed to an ALV/5000E Multiple Tau Digital Correlator and time-intensity correlation functions recorded, typically for 600 s duration
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pH-independent binding and fluorescence of DCVJOne problem frequently encountered with ThT is the pH-dependent fluorescence in amyloid fibril formation measurements
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193 K/ min (80-11 K/h) as described in Gursky et al
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1 B, solid circles) mainly reflects the formation of the protein-containing vesicles rather than intact-size disks
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In summary, chemical denaturation of discoidal lipoproteins is a complex multiphase transition whose kinetics is distinctly different from that of the thermal denaturation; similar to thermal denaturation, chemical denaturation involves protein unfolding/dissociation (Reijngoud and Phillips, 1982) and lipoprotein fusion (Fig
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This extends the results of our kinetic studies of model discoidal (Gursky et al
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Because our sample included all mature females that we encountered, we used these data to estimate the age distribution of mature females in each time period, and the age distribution of parturition during each time interval
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In one recent investigation, Donohue and her colleagues examined insurance claims from 1997 to 2000 of more than 30,000 people with depression
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Archestral and Parsons obtained this information
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